The reaction was initiated with 20 ?L of a substrate mix and halted after 15?min (HsNMT1) or 50 min (TbNMT) with 40 L of a stop answer containing 0

The reaction was initiated with 20 ?L of a substrate mix and halted after 15?min (HsNMT1) or 50 min (TbNMT) with 40 L of a stop answer containing 0

The reaction was initiated with 20 ?L of a substrate mix and halted after 15?min (HsNMT1) or 50 min (TbNMT) with 40 L of a stop answer containing 0.2 M phosphoric acid, pH 4.0 and 1.5 M MgCl2 and 1 mg?mlC1 PVT SPA beads (GE 

C-18 silica gel (40C60 m; Daiso Co

C-18 silica gel (40C60 m; Daiso Co

C-18 silica gel (40C60 m; Daiso Co., Tokyo, Japan), MCI gel CHP 20P (75C150 m, Mitsubishi Chemical Industries, Tokyo, Japan) and ATN1 Sephadex LH-20 (Amersham Pharmacia, Uppsala, Sweden) were utilized for column chromatography. of the total anthocyanins [6,7,8]; no comprehensive study has been carried out