Differences between organizations were assessed using ANOVA, and a P worth 0

Differences between organizations were assessed using ANOVA, and a P worth 0

Differences between organizations were assessed using ANOVA, and a P worth 0.05 was considered significant statistically. Results Characterization of gefitinib/PEG5k-Fmoc-NLG919 and empty micelles The blank and gefitinib-loaded PEG5k-Fmoc-NLG919 micelles were prepared utilizing a film hydration method (evaluation of Rabbit polyclonal to ADCY2 anti-lung cancer efficacy 

Kinase assays were performed in a kinase buffer (50 mM Tris-HCl, pH 7

Kinase assays were performed in a kinase buffer (50 mM Tris-HCl, pH 7

Kinase assays were performed in a kinase buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 2 mM DTT) containing 0.4 g GST-Hog1, 0.2 mM ATP, 0.1 Ci/nmol [32P]ATP, and 100 M peptide substrate, and Hog1 activity was determined as explained in the Experimental section.