IMAC has also been used to isolate histidine-tagged recombinant proteins, DNA-based aptamers, and phosphopeptides [131,278,282,284,285]
IMAC has also been used to isolate histidine-tagged recombinant proteins, DNA-based aptamers, and phosphopeptides [131,278,282,284,285]. Boronic acid and its derivatives represent another class of non-biological binding agents that have been utilized in affinity chromatography [286C289]. offers strong binding with human being IgG and IgA [162,163]. …